Figure 1.

SDS-PAGE and western blot analysis of Tc24-WT+His in-process samples. Samples were separated on 4–12% Bis-Tris gels under Non-Reduced (A) and Reduced (B) conditions. Lane 1: SeeBlue Plus Molecular Weight marker (10 µl). Lane 2: Starting Material (7.5 µl). Lane 3: IMAC eluate (7.5 µl). Lanes 4–5 QXL eluate (4 µl and 2 µl load). Lanes 6–7: Final Tc24-WT+His (4 µl and 2 µl load). Western blot detection was performed using an in house polyclonal mouse anti-Tc24 antibody at 1:2,500 followed by a goat anti-mouse IgG alkaline phosphatase conjugated secondary antibody at 1:7,500).