Figure 3.

Western blot comparison of Tc24-WT (A), Tc24-C2 (B), and Tc24-C4 (C) purified proteins. Lanes 1–3: Non-Reduced. Lane 4: SeeBlue Plus Molecular Weight Marker. Lanes 5–7 Reduced. Lanes 1 and 5: Sample before size-exclusion chromatography (SEC) 8 µg load. Lanes 2 and 6: Post SEC low load (3 µg). Lanes 3 and 7: Post SEC high load (8 µg). Detection was performed using mouse polyclonal antibody against Tc24 expressed in Pichia pastoris as primary antibody diluted 1:2,500 in PBST and an alkaline phosphatase conjugated goat anti-mouse secondary antibody diluted 1:7,500 in PBST.