Figure 5.

Stability assessment of Tc24. Western blot of different Tc24 constructs, taken after storing the proteins for 10 d at 4°C in PBS. Lane 1: Tc24-WT+His, Lane 2:Tc24-WT+His, alkylated, Lane 3: Tc24-WT, Lane 4: Tc24-C2, Lane 5: Tc24-C4. A Ponceau-stained Mark 12 protein ladder was used as a MW reference. Detection was performed using mouse polyclonal antibody against Tc24 expressed in Pichia pastoris as primary antibody diluted 1:2,500 in PBST and an alkaline phosphatase conjugated goat anti-mouse secondary antibody diluted 1:7,500 in PBST.