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. 2016 Oct 13;13(3):621–633. doi: 10.1080/21645515.2016.1242540

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Figure 8. — IFNγ measurements after homologous or heterologous stimulation of vaccinated mice by ELISA. IFNγ concentrations in supernatants from splenocytes harvested from mice vaccinated with the indicated protein combined with E6020 emulsified in AddaVaxTM and re-stimulated in vitro with homologous recombinant protein (A) or mice vaccinated with Tc24-WT No His combined with E6020 emulsified with AddaVaxTM and re-stimulated in vitro with heterologous protein as indicated (B). No statistically significant differences in antigen specific IFNγ secretion were observed. (C) IFNγ secretion from mice vaccinated with Tc24 C4 combined with E6020 in a stable squalene emulsion was significantly increased compared to mice vaccinated with Tc24 C4 (protein only) or E6020 SE alone (adjuvant only control).