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Measurement of IgG2a in vaccinated mice by ELISA. Anti-Tc24 IgG2a antibody titers in terminal serum were measured by ELISA for wild-type and mutant Tc24 constructs. Antibody titers in mice vaccinated with Tc24-C2 no His or Tc24-C4 no His were not significantly different from mice vaccinated with Tc24-WT no His.
