Table 1.
Construct | Biomass yield (g/L) | Tc24 Expression Level (mg/g biomass) | Theoretical Yield (g of Tc24 per L culture) | Starting purification load (mg) | Final purified Tc24 (mg) | Overall recovery (% of load) | Purity (%) |
---|---|---|---|---|---|---|---|
Tc24-WT | 36 | 45 | 1,630 | 183 | 83 | 45 | 93 |
Tc24-C2 | 64 | 68 | 4,354 | 155 | 85 | 55 | 98 |
Tc24-C4 | 90 | 56 | 5,062 | 192 | 100 | 52 | 98 |