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Aqy1-GFP turnover after sporulation and in germinating cells. (A) Aqy1-GFP seems to be degraded during spore maintenance. Pictures were taken from asci that had been kept on sporulation medium for 10 days. (B) Aqy1-GFP rapidly disappears from membranes upon germination. Fresh asci were transferred to YPD medium to allow germination, and GFP was monitored for 8 h. Arrows indicate green spots likely to represent a deposit of free GFP.
