Table 1. Oligonucleotides used.
Oligonucleotide | Sequence | Effect |
---|---|---|
P1 | GGTGCTGTCTGTCAATACGGCACATAAAGTAACATGTAATTAACTATAACCAGTGAATTCCCGGGGATCC | Replacement AQY1::HIS3 |
P2 | CGAGTATTATAACATTAAGTGCTAGTGAGCGAGAAATAAAGAAAAGGAGGTGACCATGATTACGCCAAGC | Replacement AQY1::HIS3 |
P3 | GAACGATACCGACAAGCAAC | Confirming deletion |
P4 | ATAAACTGGGCACACCAAG | Confirming deletion |
P5 | GAGAGTGCGTTCAAGGCTCTTGCG | Confirming HIS presence |
P6 | TCGCTCACTAGCACTTAATGTTATAATATTCGGCAAAAACTCTAAAGGTGAAGAATTATTCACTG | Insertion GFP |
P7 | AGGGATATTAAAAACACTAATTACCTCAGTAGTATGGATGGCATAGGCCACTAGTGGATCTG | Insertion GFP |
P8 | GGCGTGAATGTAAGCGTGACAT | Confirming GFP |
P9 | ACATTAAGTGCTAGTGAGCG | Confirming insertion |
P10 | CTATCAACTTTCGATGGTAGG | Probe 18S |
P11 | TATGGTTAAGACTACGACGGT | Probe 18S |
P12 | TTTGAACACGGCATTAGTCAG | Amplification of HIS3 |
P13 | CTCGTTCAGAATGACACGTATAG | Amplification of HIS3 |
Underlined residues match plasmid sequence.