Table 1. Oligonucleotides used.
| Oligonucleotide | Sequence | Effect |
|---|---|---|
| P1 | GGTGCTGTCTGTCAATACGGCACATAAAGTAACATGTAATTAACTATAACCAGTGAATTCCCGGGGATCC | Replacement AQY1::HIS3 |
| P2 | CGAGTATTATAACATTAAGTGCTAGTGAGCGAGAAATAAAGAAAAGGAGGTGACCATGATTACGCCAAGC | Replacement AQY1::HIS3 |
| P3 | GAACGATACCGACAAGCAAC | Confirming deletion |
| P4 | ATAAACTGGGCACACCAAG | Confirming deletion |
| P5 | GAGAGTGCGTTCAAGGCTCTTGCG | Confirming HIS presence |
| P6 | TCGCTCACTAGCACTTAATGTTATAATATTCGGCAAAAACTCTAAAGGTGAAGAATTATTCACTG | Insertion GFP |
| P7 | AGGGATATTAAAAACACTAATTACCTCAGTAGTATGGATGGCATAGGCCACTAGTGGATCTG | Insertion GFP |
| P8 | GGCGTGAATGTAAGCGTGACAT | Confirming GFP |
| P9 | ACATTAAGTGCTAGTGAGCG | Confirming insertion |
| P10 | CTATCAACTTTCGATGGTAGG | Probe 18S |
| P11 | TATGGTTAAGACTACGACGGT | Probe 18S |
| P12 | TTTGAACACGGCATTAGTCAG | Amplification of HIS3 |
| P13 | CTCGTTCAGAATGACACGTATAG | Amplification of HIS3 |
Underlined residues match plasmid sequence.