Table 1. Incorporation of 35S methionine.
| Ratio of cpm (t = n/t = 0)
|
||
|---|---|---|
| Condition | WT | KO |
| Unstimulated | ||
| 1 min | 1.21 | 1.05 |
| 2 min | 1.17 | 1.21 |
| 5 min | 1.61 | 1.19 |
| Stimulated | ||
| 1 min | 1.35 | 1.06 |
| 2 min | 1.62 | 1.14 |
| 5 min | 2.06 | 1.12 |
Synaptoneurosomes from WT and KO mice were incubated with 35S methionine, and incorporation into polypeptides (cpm) was measured in samples removed at short intervals from parallel unstimulated and DHPG-stimulated aliquots. WT synaptoneurosomes exhibit a significant (P < 0.05) increase in 35S methionine incorporation (cpm) 5 min after DHPG stimulation, compared with WT unstimulated samples. KO synaptoneurosomes do not exhibit this increase after stimulation (all samples normalized to cpm at t = 0).