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. 2017 Mar 23;2(6):e91020. doi: 10.1172/jci.insight.91020

Figure 6. 3M-052 enhances and accelerates activation of early life PnPS-specific B cells.

Figure 6

(A and B) Antipneumococcal polysaccharide (PnPS) IgG/IgM-producing rhesus naive and memory B cell quantification. Higher frequencies of PnPS-specific B cells were noted in the (PCV13 + 3M-052) animals vs. the PCV13 group (n = 3–4). (C) Coadministration of 3M-052 with PCV13 to newborn rhesus macaques dramatically accelerated the transition of anti-PnPS B cells from naive to memory phenotype. Antipneumococcal polysaccharide (PnPS) IgG-producing naive (CD27–) and memory (CD27+) B cells were measured by flow cytometry. At day of life 28 (DOL28), the switch from naive to memory phenotype occurred earlier in (PCV13 + 3M-052)– vs. PCV13 only–immunized animals. Nonspecific polysaccharide in vitro activation in the control conditions (i.e., saline or 3M-052 alone) or specific CRM-197 treatment of B cells from all treatment groups never exceeded approximately 1.0%. For comparison at individual time points, the unpaired Mann-Whitney test was applied, *P < 0.05, **P < 0.01. Results represent mean ± SEM (n = 4 per group).