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. 2004 Dec 2;101(50):17510–17515. doi: 10.1073/pnas.0405313101

Fig. 5.

Fig. 5.

Parkin increases the number of phosphorylated α-synuclein inclusions. Sections from the substantia nigra of rats expressing A30P α-synuclein and parkin were immunostained with the Pser129 Ab specific for phospho-Ser-129 of α-synuclein that selectively and extensively accumulates in human Lewy bodies. (A) A very weak Pser129 staining was observed in the noninjected side corresponding to the physiological level of phosphorylated rat α-synuclein. (B) The substantia nigra of animals overexpressing either A30P/YFP or A30P/parkin revealed the presence of numerous hyperphosphorylated inclusions reminiscent of Lewy bodies. (C) Pser129-positive neurites were occasionally observed in animals overexpressing A30P/parkin. (D) Triple staining with Pser129 Ab (green), LB509 human α-synuclein specific Ab (red), and TOPRO-3 nuclear marker (blue) shows that phosphorylated inclusions abundantly contain human α-synuclein. (E) The number of neurons containing Pser129-positive inclusions were quantified in the substantia nigra of rats expressing A30P/YFP or A30P/parkin. Values refer to means ± SEM; n = 6 animals per group; *, P < 0.05; **, P < 0.005. (Scale bars: A and B, 150 μm; C, 40 μm; D, 10 μm.)