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. 2017 May 1;393:68–75. doi: 10.1016/j.canlet.2017.02.018

Fig. 5.

Fig. 5

Saporin-Alexa-Fluor488®subcellular localisation & redistribution upon excitation of TPPS2a. Fluorescence microscopy of Saporin-Alexa Fluor488® in PC3 cells. (A) Saporin-Alexa Fluor488® (400 nM) prior to illumination with 405 nm laser. (B) Saporin-Alexa Fluor488® (400 nM) post exposure to 405 nm laser. (C) Saporin-Alexa Fluor488® (400 nM) and TPPS2a (0.4 μg/ml) prior to illumination with 405 nm laser. (D) Saporin-Alexa Fluor488® (400 nM) and TPPS2a (0.4 μg/ml) post exposure to 405 nm laser. (E) Saporin-Alexa Fluor488® (400 Nm). (F) LysoTracker® Red DND-99 (75 nM). (G) Merged Saporin-Alexa Fluor488® (E) & LysoTracker® Red DND-99 (F). Scale bars shown as 50 μm.