Fig. 1.
Response of the three probes to air oxygen. Each figure displays the dynamics of 60 measured emission spectra (one spectrum per minute; Em=510 nm) in the excitation wavelength range of 350–500 nm. First (0 min) and last spectra (60 min) are displayed in green and red, respectively. The probes were added to PBS buffer at a final concentration of 0.2 µM (A–E) or expressed in E. coli (F). Measurements were either done under aerobic conditions (A–C) or under fully anaerobic conditions (D). One volume of aerobic buffer corresponding to 100 µM O2 was added after measurement of the first spectrum (E).