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. 2004 Dec 1;101(50):17468–17473. doi: 10.1073/pnas.0406142101

Fig. 3.

Fig. 3.

TGF-β induction of 15-PGDH. (A) Northern analysis of 15-PGDH in TGF-β-treated (10 ng/ml) Vaco-330 colon adenoma cells at 48 and 72 h of incubation, with corresponding ethidium bromide staining of the 28S ribosomal subunit shown below. (B) Northern analysis of 15-PGDH in TGF-β-treated (10 ng/ml, 48 h) Vaco-410 colon cancers cells transfected with wild-type RII (V410/RII) or control empty vector (V410/Neo). (C) Real-time PCR of 15-PGDH induction by TGF-β (10 ng/ml) in FET colon cancer cells. (D) Western analysis of 15-PGDH protein induction by TGF-β (10 ng/ml) in FET colon cancer cells. (E) Induction of 15-PGDH enzyme activity (pmol/min per mg protein) in TGF-β-treated (10 ng/ml) FET cells. Values are averages of duplicate determinations. (F) Northern analysis of 15-PGDH induction by TGF-β (10 ng/ml, 18 h) in cycloheximide treated (CHX+) versus untreated (CHX–) FET cells.