Fig. 4.

Excitation and emission spectra of hen egg white lysozyme (HEWL) is closely associated with aggregation. (A) Excitation and emission spectra of hen egg white lysozyme (HEWL) at various time-points during the aggregation process (at 0, 2, 4, 6, 8, 11 days; solid line: excitation, dashed line: emission). The total fluorescence intensity increases with aggregation time. The excitation and emission maxima are at 355 nm and 440 nm respectively. (B) ThT binds to the HEWL fibrils formed and displays its typical excitation and emission maxima at 445 nm and 486 nm respectively (solid line: excitation, dashed line: emission).