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Stereo diagrams of the structures of NgbCO (red) and unligated metNgb (magenta) in ribbon representation. (A) Overall structures, the distal histidine (His-64), the proximal histidine (His-96), Phe-106, the heme group, and loops EF and FG are highlighted. Green regions indicate the positions of the two point mutations, Cys-55–Ser and Cys-120–Ser. (B) Close-up view of the active site, also including Tyr-44 and Phe-106.
