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FTIR absorbance difference spectra of the stretching bands of hemebound CO in WT Ngb at pH 7.3 (solid line) and pH 5.3 (dashed line) (A), mutant Lys-67–Leu at pH 7.3 (solid line) and pH 5.5 (dashed line) (B), and mutants His-64–Leu (pH 7.4) and His-64–Leu–Lys-67–Leu (pH 7.3) (C). Difference spectra were calculated from absorbance spectra taken before and after illumination with 532-nm light at 3 K.
