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Microtubule binding properties of Klp1. (A) Western blots of supernatants (S) and pellets (P) after incubation of dialyzed NaCl extract (HSE) with extracted axonemes (ExAx). Most of the Klp1 remains in the supernatant even in the presence of AMP-PNP (PNP). (B) Binding of Klp1 to reassembled brain microtubules. Klp1 binds in the presence of AMP-PNP (PNP), but not ATP, and is released from the AMP-PNP pellet by subsequent ATP treatment (P+A).
