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. 2017 Mar 21;12(3):e0174224. doi: 10.1371/journal.pone.0174224

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© 2017 Marti et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — H295R cells were transfected with human SIRT1, SIRT3 and SIRT5, respectively and grown under normal conditions (GM) or under resveratrol (RSV) (5μM) treatment. Transfection with empty vector pcDNA3 was used as a control. A. Representative steroid profile obtained from transfected H295R cells. Steroid production was labeled with [3H]-pregnenolone for 90min and extracted steroids were resolved by TLC. B. Quantitative analysis of steroid production showed no alterations after overexpression of sirtuins. Data are indicated as the mean ± SD of three independent experiments. TLC, thin layer chromatography; Prog, progesterone; Δ4A, androstenedione; Preg, pregnenolone; 17OHPreg, 17-hydroxypregnenolone; 17OHP, 17-hydroxyprogestertone, 11DOC, 11-deoxycortisol.