Figure 4.

AAT inhibits activation of coagulation after intraportal transplantation. A: Reduced TF expression in AAT-treated grafts compared with control (Ctrl) grafts. Immunohistochemistry staining of TF (green) in transplanted islets (red for insulin) from AAT or Ctrl mice. Arrows point to TF⁺ cells. B: Relative fluorescence intensity of TF divided by intensity of insulin in grafts from AAT or Ctrl mice. C: Change of plasma TAT levels in AAT and Ctrl mice at 3 and 6 h PT. D: Fibrinogen deposition in transplanted islets 6 h after intraportal transplantation in Ctrl and AAT mice. Serial paraffin sections of an islet graft were stained with anti-insulin (left) and anti-fibrinogen (right). The dashed line outlines islet grafts based on the insulin staining. The arrow indicates fibrin deposition on islet graft. Scale bars = 100 μm. Data are expressed as mean ± SD. *P < 0.05 AAT vs. Ctrl by Student t test.