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. 2017 Mar 21;12(3):e0174249. doi: 10.1371/journal.pone.0174249

Table 1. Primers used for PCR cloning and real-time PCR analysis of gene expression.

Gene Primer name Primer sequence (5´-3´) Application
TACI SS-TACI-F GCTCAGTGTCACTGCATGAGAGGC Cloning
SS-TACI-R ACACGTCTCTGTGGGGCTGG Cloning
TACI-3-RACE GTGTCTGGCTCTGCTGCTGTTGACC 3´RACE
TACI-3-RACE-nest GTGCTGCTGAGGAGGGCCAGTGCC 3´RACE
RT-TACI-F GCATCGAGTACTGTGCTTCTCTAGG Real time PCR
RT-TACI-F AAGTCAGGCTGTTGGGTCTTACATT Real time PCR
BCMA SS-BCMA-F ATGTCAGAAGGACAGTGTGGACTGG Cloning
SS-BCMA-R CTGTGTGGTTAAAGATCTGTACTGTCTTGG Cloning
RT-BCMA-F ATGTCAGAAGGACAGTGTGGACTGG Real time PCR
RT-BCMA-R CGGCTCTGGGGCTTTGCTCT Real time PCR
BAFF-R BAFFR-3-RACE- CCTCGGTGTGTGGCTCTCTTGGGG 3´RACE
BAFFR-3-RACE-nest GGGAGCATTGGATCCCACTTCCTGC 3´RACE
RT-BAFFR-F TGTCTGGATATCAATGGTCGTCATA Real time PCR
RT-BAFFR-R CTTTAGCTGGAGGGTTAAGTCTTGC Real time PCR
BAFF RT-BAFF-F ATGTTTGATGCTTATTCTGGCAGGT Real time PCR
RT-BAFF-R TGGGACTGTGTCTTGACTGTGTGTA Real time PCR
APRIL RT-APRIL-F CACAGACATACACAATGGAATGGAA Real time PCR
RT-APRIL-R TGTGATGACAGAGGAACAAGATGAA Real time PCR
BALM RT-BALM-F TGGAGGTACAGTAGTTCAGCAGTCG Real time PCR
RT-BALM-R ACTATCCAAGGAATCACCGTCACAT Real time PCR
IgMtotal RT-IgMtotal-F TGCGTGTTTGAGAACAAAGC Real time PCR
RT-IgMtotal-R GACGGCTCGATGATCGTAAT Real time PCR
IgMsec RT-IgMsec-F CCTTAACCAGCCGAAAGGG Real time PCR
RT-IgMsec-R TGAGGTTCTATCAATGGTTCTC Real time PCR
IgT RT-IgTtotal-F AACATCACCTGGCACATCAA Real time PCR
RT-IgTtotal-R TTCAGGTTGCCCTTTGATTC Real time PCR
IgD RT-IgDtotal-F AGCTACATGGGAGTCAGTCAACT Real time PCR
RT-IgDtotal-R CTTCGATCCTACCTCCAGTTCCT Real time PCR
EF-1α RT-EF1α-F GATCCAGAAGGAGGTCACCA Real time PCR
RT-EF1α-R TTACGTTCGACCTTCCATCC Real time PCR