Table 1. List of genes with their forward and reverse primer sequences used for the gene expression analysis by real- time quantitative PCR.
Gene | Forward primer sequence (5’-3’) | Reverse primer sequence (5’-3’) | Size(base pair) |
---|---|---|---|
GAPDH | GGTGGTCTCCTCTGACTTCAACA | GTTGCTGTAGCCAAATTCGTTGT | 116 |
CyclinD1 | AACTACCTGGACCGCTTCCT | CCACTTGAGCTTGTTCACCA | 204 |
Bcl2 | GGATGCCTTTGTGGAACTG | AGCCTGCAGCTTTGTTTCAT | 231 |
Bax | TTTGCTTCAGGGTTTCATCC | CAGTTGAAGTTGCCGTCAGA | 246 |
TOP2A | GGGTTCTTGAGCCCCTTCAC | CCAATGTAGGTGTCTGGGCG | 185 |
MLH1 | TGGGACGAAGAAAAGGAATG | GATCAGGCAGGTTAGCAAGC | 250 |
MYH | CAGAGGCTTTGAAGGCTACC | TGCAGCATGACCTCTGAGAC | 458 |
RAR-β 2 | CCACACCTAGAGGATAAGCACT | GTGGTACCCTGATGATTTGTCC | 326 |
MBD4 | CAACTGCTCACCAACCAGGA | AAAAGGTGACCGAGGAGGTG | 184 |
MSH2 | GCCATTTTGGAGAAAGGACA | CTCACATGGCACAAAACACC | 228 |
PARP1 | GCCCTAAAGGCTCAGAACGA | AAGGCACTTGCTGCTTGTTG | 112 |
OGG1 | GACAAGAGCCAGGCTAGCAG | CTCTTGGAAGTGGGAGTCCA | 126 |