Table 4. Neutralization of BoNT/F subtypes in the mouse neutralization assay by HBAT of an equimolar combination of mAbs 6F5.1, 6F11 and 6F13.

	mAbs 6F5.1+6F11+6F13				Equine antitoxin (HBAT)
BoNT/F subtype	F1	F2	F4	F7	F1	F2	F4	F7
Experimentally-determined LD50 of 1 μg of toxin	20000	2000	480	11000	66001	2000	480	11000
Antibody Dose (μg)	Mice surviving/ mice studied
0.5	1/10	0/10	0/10
1.0	8/10		0/10
2.0	8/10		1/10
5.0	10/10	0/10	10/10	2/10
10	10/10			8/10	1/10
20				10/10	2/10
50		7/10	10/10	9/10	10/10	0/10	0/10	0/10
100		7/10			10/10	1/10	5/10	0/10
150					10/10	2/10	10/10	0/10
300					10/10	4/10		0/10
ED50 (ug)3	0.5–1.0	20–50	2–5	5–10	20–50	>3002	100	>>3002

¹ BoNT/F1 dose reduced as 50% survival not achieved at maximal HBAT dose with 20,000 LD₅₀ challenge.

² 300 μg of HBAT protected 9 of 10 mice from 1,000 LD₅₀ BoNT/F2 and 344 LD₅₀ of BoNT/F7 challenge.

³ The ED₅₀ is the mAb dose at which greater than 50% of the mice studied survived