Fig 6. Lzts2 deletion increases cellular proliferation and nuclear ß-catenin in the mouse prostate.

(A-C). Cellular proliferation was examined by immunostaining for Ki-67. Prostate sections isolated from Lzts2^LoxP/LoxP:PB-Cre4, Pten^loxP/Wt:PB-Cre4, and Lzts2^LoxP/LoxP -Pten^loxP/Wt:PB-Cre4 mice were stained for Ki-67. (D) A total of 1000 epithelial cells in each lesion from three different lesions from three mice of each genotype were evaluated for Ki-67 immunoreactivity. (E) Mouse embryonic fibroblasts (MEFs) were prepared from different genotype embryos at E10.5. Either whole cell lysates or nuclear extracts were isolated from different genotype MEFs and analyzed by Western-blotting assays for either ß-catenin (ß-cat), tubulin, or PCNA. (F-H) Representative H&E and ß-catenin staining of Prostate tissues from the three different genotype mice is shown. Boxes highlight strong nuclear ß-catenin staining observed with conditional LZTS2 deletion (F2, H2). “*” or “**” means P<0.05 or <0.01, respectively.