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Growth curves of BL21-DE3-ΔE1 harboring pRSETB-Y-EI cells in Neidhardt medium at the indicated ribose concentrations. In stationary phases, the percentages of punctate cells were as follows: in 1.89 mM ribose, 5–10%; 3.78 mM, 15%; 5.67 mM, 30%; 7.56 mM, ≈35%; 30 mM, >80%. During the growth phases, no punctate cells were observed; only a diffuse distribution of fluorescence was seen.
