Figure 2.

(A) Reactive oxygen species (ROS) are involved in angiotensin II (Ang II)-induced low-density lipoprotein (LDL) transcytosis across human umbilical vein endothelial cells (HUVECs). HUVECs were pretreated with methyl-β-cyclodextrin (MβCD) or dithiothreitol (DTT) for 30 min, and then stimulated with fluorescein isothiocyanate (FITC)-LDL (and/or LDL) and Ang II for 24 h. After 24 h, samples were collected from the outer chambers and further dialyzed against PBS to remove the free FITC. (B) The relative fluorescence was measured via a fluorescence spectrophotometer. The amount of LDL transcytosis was normalized to that obtained in the control group. ^*P<0.05 vs. control; ^#P<0.05 vs. Ang II.