Figure 5.

Effect of hypoxic preconditioning on the wound healing properties of adipose-derived stem cells (ASCs). Conditioned and dialyzed StemPro (StemPro CD) from ASCs cultured either under normoxic or hypoxic conditions, was used in the scratch assay on keratinocytes. (A) Representative micrographs from each condition, showing the original wound and the wound after 24 h. Scale bar denotes 300 µm. The circular shapes in the upper right image are air bubbles from the scratching procedure. (B) Quantitative analysis of relative wound closure at different time points. First, the wound closure was determined as the wound area at a given time relative to the original wound area, after which the relative wound closure was calculated as wound closure in conditioned medium divided by the wound closure in EpiLife. Data are presented as the means ± SEM (n=6). The data from normoxic and hypoxic media was compared showing a statistical significant difference after 24 h, ^*p<0.01.