Figure 4.

Effect of lipopolysaccharide (LPS) and neutrophil extracellular trap (NET) treatment on the production of interleukin (IL)-6 and tumor necrosis factor (TNF)-α by J774 cells. J774 cells (2×10⁴ cells) were treated with LPS (10 ng/ml), NETs (1.7×10⁵ cell equivalents) or their combinations in 100 µl RPMI-1640 medium for 24 h. Thereafter, the supernatants were recovered for the assays of (A) IL-6 and (B) TNF-α. Data show the means ± standard deviation of 3 separate experiments. Values are compared between without and with LPS. ^***P<0.001.