Figure 5.

Involvement of neutrophil extracellular trap (NET)-associated DNA in the NET/lipopolysaccharide (LPS)-induced interleukin (IL)-1β production. J774 cells (2×10⁴ cells) were treated with LPS (10 ng/ml) and NETs (1.7×10⁵ cell equivalents) for 24 h in 100 µl RPMI-1640 medium in the absence or presence of nucleases [(A) deoxyribonuclease I (DNase I); (B) micrococcal nuclease (MNase)]. Thereafter, the supernatants were recovered for the assays of IL-1 β. (C) Furthermore, J774 cells were treated with LPS (10 ng/ml) and calf thymus (CT)-DNA (1 and 10 µg/ml) for 24 h, and then the supernatants were recovered for the assays of IL-1β. Data shows the means ± standard deviation of 3-6 separate experiments. Values are compared between the NET/LPS treatment in the absence and presence of (A and B) nucleases, or LPS alone and (C) CT-DNA/LPS treatment. ^**P<0.01, ^***P<0.001.