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. 2017 Feb 6;39(3):527–538. doi: 10.3892/ijmm.2017.2880

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Isopsoralen (ISO) stimulates bone-derived marrow mesenchymal stem cell (BMSC) differentiation into osteoblasts in vitro. ISO (0, 5, 10 or 20 µM) was added to primary mouse BMSCs under osteogenic differentiation conditions for 2 weeks. (A) Osteocalcin (OCN) immunocytochemistry and (B) runt-related transcription factor 2 (RUNX2) immunofluorescence were performed to examine the expression of osteoblast-specific proteins following treatment with ISO. (A) ISO increased OCN expression (brown) in BMSCs in a dose-dependent manner, with more pronounced effects at 20 µM ISO (C). (B) ISO dose-dependently enhanced RUNX2 (red) activity in BMSCs, particularly at a concentration of 20 µM (D). ^*P<0.05, ^**P<0.01 and ^***P<0.001 compared to the group without ISO (Con, control). Columns represent the means ± SD from 9 wells/group (C and D).