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. 2017 Feb 6;39(3):527–538. doi: 10.3892/ijmm.2017.2880

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Isopsoralen (ISO) supresses the adipogenic and promoted osteogenic differentiation of bone-derived marrow mesenchymal stem cells (BMSCs) by inhibiting the activation of the mTORC1 pathway in vitro. (A) ISO altered the expression of osteocalcin (OCN) (G) and runt-related transcription factor 2 (RUNX2) (H) during the osteoblastic differentiation of cultured BMSCs for 7 days. Under adipogenic differentiation conditions for 1 week, ISO inhibited peroxisome proliferator-activated receptor γ (PPARγ) (C and K) and CCAAT/enhancer binding protein β (C/EBPβ) expression (C and L). PPARγ (E and O) and C/EBPβ (E and P) expression was markedly decreased in the presence of ISO at day 14 in a dose-dependent manner (O and P). mTORC1 signaling was down-regulated in (B) the osteoblastic differentiation process and (D and F) under adipogenic conditions. (B and I) Eukaryotic translation initiation factor 4E-binding protein 1 (4E/BP1; Thr37/46) expression was upregulated and (B and J) phospho-S6 ribosomal protein (P-S6; S235/236) expression was decreased 2 weeks following osteogenic induction. The expression of (M and Q) 4E/BP1 (Thr37/46) and (N and R) P-S6 (S235/236) in adipogenic medium at (M and N) 7 days or (Q and R) 14 days was similar to that in BMSCs cultured under osteogenic conditions for 7 days. ^*P<0.05, ^**P<0.01 and ^***P<0.001 compared to the group without ISO (Con, control). Columns represent the means ± SD from 9 wells/group (G–R).