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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Nat Protoc. 2012 Dec 13;8(1):66–74. doi: 10.1038/nprot.2012.144

TABLE 1.

Troubleshooting table.

Step Problem Possible reason Solution
9 Air bubbles Solution not prewarmed Ensure solution is prewarmed to at least 40–50 °C
Air pockets in catheters Agitate the catheters as they are flushed to ensure all air is purged.
17 Arterial leak Pierced or punctured wall An arterial leak can be fixed by suturing the vascular wall to the catheter downstream of the leak (Supplementary Fig. 2 and Supplementary Video 2)
21 Arterial rigor Excessive delay Surgical processes improve over time, decreasing the delay, but as a short-term solution the umbilical vein and artery can be separated after the uterine artery has been cannulated, reducing umbilical contraction and stiffening
24 Venous collapse Elevated negative pressure Lowering negative pressure and removing the complete vascular seal can improve perfusion efficiency, but it does lead to reduced collection rate
Vascular wall suction Use of a larger-diameter catheter can prevent possible wall suction, as the larger diameter is more difficult to obstruct