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. 2017 Feb 17;39(4):927–935. doi: 10.3892/ijmm.2017.2893

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of transmembrane protease serine 4 (TMPRSS4) knockdown on cell proliferation in MDA-MB-468 and MDA-MB-231 cell lines using EdU assay. (A and C) Representative images after transfection with siRNA in MDA-MB-468 and MDA-MB-231 cells (magnification, x100). (B and D) The percentage of EdU-positive cells, calculated by the EdU-labeled cell (red) number compared to total cell number (DAPI, blue), was defined as the proliferation rate. Cells with siRNA transfection showed significantly fewer proliferating cells than the negative control group. ^**P<0.01 and ^***P<0.001. NC, negative control.