Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Feb 17;39(4):927–935. doi: 10.3892/ijmm.2017.2893

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Knockdown of transmembrane protease serine 4 (TMPRSS4) regulated the expression of epithelial-mesenchymal transition (EMT) related genes. (A and B) RT-qPCR demonstrated that EMT markers of E-cadherin and vimentin were upregulated and downregulated respectively after TMPRSS4 knockdown in MDA-MB-468 and MDA-MB-231 cells. For the expression of key EMT transcriptional factors, claudin-1 and slug were upregulated and downregulated after transfection with siRNA, respectively. However, expression of ZEB1 did not show significant change. (C-F) Transfection efficiency and similar results were further observed in western blot analysis. β-actin was an internal control. ^*P<0.05, ^**P<0.01 and ^***P<0.001. NC, negative control; EMT, epithelial-mesenchymal transition.