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Matrigel tube formation analysis in vivo. (A) Gross anatomy of freshly removed Matrigel plugs with conditional medium 1 week after implantation. (B) Masson trichrome staining of plug sections of EBM-2, CdM(EBM-2) hAEC and CdM(EBM-2) hAMSC-treated mice. CdM from both amniotic cells promoted blood vessels formation in vivo. Scale bar, 50 μm. CdM, conditioned medium; EBM-2, Endothelial Basal Medium-2; hAEC, human amniotic epithelial cell; hAMSC, human amniotic mesenchymal stem cell.
