Figure 10.

Changes in paraoxonase and cholesteryl ester transfer protein (CETP) activities in high-density lipoprotein 3 (HDL₃) at 0, 4 and 8 weeks after policosanol consumption. Error bars indicate the SD from three independent experiments with duplicate samples. (A) Paraoxonase activity. Equally diluted HDL₃ (20 µl, 2 mg/ml) was added to 230 µl of paraoxonethyl (cat. no. D-9286; Sigma-Aldrich)-containing solution [90 mM Tris-HCl/3.6 mM NaCl/2 mM CaCl₂ (pH 8.5)]. (B) CETP activity. The same amount of protein in each individual HDL₃ fraction (50 µg of total protein) was utilized as a source of CETP. Human low-density lipoprotein (LDL) (0.25 mg/ml of protein) and rHDL containing [³H]-cholesteryl oleate were used as a CE-acceptor and CE-donor, respectively.