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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Immunol. 2017 Feb 17;198(7):2699–2711. doi: 10.4049/jimmunol.1601202

Figure 4.

Figure 4

Itk−/− CTLs have normal adhesion and actin ring formation during immunological synapse formation. (A) Adhesion after 20 minutes conjugation of previously activated WT (black) or Itk−/− (grey) CTLs to LPS-activated WT B cells pulsed with 1μM OVA257-264 or unpulsed B cells as a control. Graph represents mean ± SD of percent CD8+ target+ cells in total CD8+ events. Data are representative of one of greater than three experiments. (B) Representative images of maximum projections of WT or Itk−/− CTLs in conjugate pairs with LPS-activated WT B cell targets pulsed with 1μM OVA257-264 (first and third columns, respectively). 1μm slice of reconstructed z stacks rotated in the yz plane (second and fourth columns). Nuclei (blue), CD8 (red), actin (green). Scale bars = 5μm. (C) Quantification of actin ring formation at the immunological synapse between WT (black) and Itk−/− (grey) CTLs and LPS-activated WT B cell targets. Bars represent mean ± SEM from more than three independent experiments (WT total n=105, Itk−/− total n=82).