(A) Representative flow cytometry plots from the thymus of C57BL/6 ,
Nod1−/−,
Nod2−/−, and
Nod1−/−Nod2−/−mice
(labeled above) stained for CD4 and CD8a. Numbers in plots represent percentages
of depicted populations. Total proportions (panel B) and numbers (panel C) of
thymocyte subpopulations of C57BL/6 (black),
Nod1−/−(grey),
Nod2−/−(grey hatched), and
Nod1−/−Nod2−/−
mice (white) are shown. Bars represent mean +/− SD. Statistical analysis
with paired t tests was performed. Data represent three animals per group.