Skip to main content
. Author manuscript; available in PMC: 2018 Mar 7.
Published in final edited form as: Pigment Cell Melanoma Res. 2017 Mar 7;30(2):203–218. doi: 10.1111/pcmr.12564

Figure 3. B16F0 exosomes contain mRNAs that are differentially expressed relative to parental cells.

Figure 3

(a) The distribution in RNA contained within the parental B16F0 cells (top panel) and B16F0 exosomes (bottom panel) was quantified using microfluidic electrophoresis (Agilent 2100 Bioanalyzer). (b) Hierarchical clustering of mRNA detected above background (p < 0.01) from four B16F0 exosomes and four B16F0 cell samples using Affymetrix Exon-level cDNA microarrays. (c) The overall distribution in exosome versus cellular abundance of mRNAs (gray curve) was deconvoluted into three normally distributed populations: mRNAs enriched in cells (red curve 67% of total), mRNAs equally distributed between cell and exosomes (black curve 30% of total), and mRNAs enriched in exosomes (blue curve 3% of total). (d) Using a more stringent gene call (p < 1e-9), a Venn diagram summarizes the number of mRNA genes differentially expressed between cell and exosome samples.