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. Author manuscript; available in PMC: 2018 Mar 7.
Published in final edited form as: Pigment Cell Melanoma Res. 2017 Mar 7;30(2):203–218. doi: 10.1111/pcmr.12564

Figure 8. An increase in PTPN11 inhibited the proliferation of CTLL-2 cells in response to IL-2.

Figure 8

(a) At the indicated time points, PTPN11 was assayed in CTLL-2 cells following a 40 hour recovery in complete medium after transient transfection with expression plasmids containing either the gene for GFP (blue circles and solid line) or Ptpn11 (red triangles and dotted line). (b, c) CTLL-2 T cells were transfected with Ptpn11 plasmids (red curves), GFP plasmids (blue curves), or no plasmid control (green curves), recovered for 40 hours in complete medium, stained using CellTrace Violet (CTV), and then stimulated with 2000 U/ml IL-2. Following 48 hours of stimulation with IL-2, the levels of PTPN11 and dilution of CellTrace Violet in live cells were assayed by flow cytometry, where at least 20,000 events were acquired. The results are representative of at least three replicates. (b) Scatter plots summarized the intensity of PTPN11 staining versus CellTrace Violet staining for cells transfected with a GFP plasmid (dots). Similar results for untransfected CTLL-2 cells and cells transfected with a PTPN11 plasmid are shown as a single contour that encloses 95% of the population (green oval: untransfected CTLL-2 cells, blue oval: GFP-transfected CTLL-2 cells, red oval: PTPN11-transfected CTLL-2 cells). (c) Histograms of CellTrace Violet staining in three biological replicates of untransfected (green curves), GFP-transfected (blue curves), and PTPN11-transfected (red curves) observed after 48 hours were compared to CTLL-2 cells fixed at 0 hours (gray shaded curve).