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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Immunol. 2017 Feb 17;198(7):2796–2804. doi: 10.4049/jimmunol.1602076

FIGURE 5.

FIGURE 5

Inflammatory monocytes are activated prior to egress from the bone marrow. Bone marrow (BM) from uninfected mice (UI; grey bars) or mice fed 108 CFU of Lm SD2710 (black bars) was analyzed 2 dpi (n=6). (A) CD64 expression on Ly6Chi monocytes (Mo) and common monocyte progenitors (cMoP) (56). (B) Mean percentage (±SD) of MHC-II+ Ly6Chi Mo and cMoP. (C) BM harvested from both femurs and tibias was plated and L. monocytogenes CFU were determined 2 dpi (n=6). (D) Mean concentration (±SD) of IL-12 and IL-18 in mouse sera (n=4). (E) Mean concentration of IFNγ (±SD) in the bone marrow without in vitro stimulation (n=4). (F) Representative contour plots of bone marrow cells showing the percentage of IFNγ+ cells. Bar graphs to the right indicate mean number (±SD) of IFNγ+ Ly6Cint, T cells, or NK cells (n=4). Two-tailed Mann-Whitney tests were used for statistical analysis.