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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Struct Biol. 2016 Dec 21;197(3):312–321. doi: 10.1016/j.jsb.2016.12.010

Figure 5. Nanometer-scale comparison of the tips of FLPs of ARPC3−/− fibroblasts and of filopodia of their wild-type isogenic control cells. Each example shows a 3.8 nm thick slice through a three-dimensional cyro-tomogram in grayscale with a paired three-dimensional rendering of red actin filaments and blue plasma membrane traced in boxed areas of the reconstructions. Bars are 200 nm. Raw tomogram slices of the traced regions are shown to the right.

Figure 5

A. Wild-type filopodium tip with ‘terminal cone’ geometry.

B. Wild-type filopodium tip with parallel filaments. Figure S3 shows complementary information.

D, E. Bulbous ARPC3−/− FLP tips with densely packed actin filaments. Occasionally, an FLP tip interacts with the shaft of another FLP as in (E).