Figure 6. OXPHOS inhibition reduces T_H17 cytokine production.

(A–F) In vivo (red)- or in vitro (blue)-differentiated T_H17 cells were treated for one hour with DMSO or the indicated concentration of oligomycin and then stimulated with PMA/ionomycin. IL-17 expression was analyzed by FACS (A). The production of IL-17a, GM-CSF, TNF, and IL-21 in cell-free supernatant from triplicate cultures was evaluated by ELISA (B–F). (A–F) Results are expressed as percentage of DMSO-treated cells. (A) For in vivo T_H17, each dot represents an individual mouse; for in vitro T_H17, an independent cell culture. (A–F) Representative of three independent experiments; *p < 0.001; N.S., not significant (A: paired Student’s t-test; B–F: unpaired Student’s t-test); error bars, mean ± SEM.