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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Immunol. 2017 Feb 24;198(7):2854–2864. doi: 10.4049/jimmunol.1602006

Figure 8. NADPH oxidase-mediated ROS production in the BM is critical for proliferation of myeloid progenitors during acid-elicited acute lung injury.

Figure 8

(A) Neutrophil recruitment to the lungs during acid-elicited acute lung injury. The experiments were conducted 48 hr after the acid treatment. Data shown are means ± SD of n=5 mice. (B) BM extracellular ROS measured using the Amplex® Red assay. Data shown are means ± SD of n=5 mice. * p<0.01 versus PBS treated mice. (C) Acid-induced elevation of myeloid progenitor subset depends on phagocyte NADPH oxidase. Flow cytometry-based lineage analysis was conducted in WT and CGD mice 48 hr after acid instillation. Data shown are means ± SD of n=5 mice. * p<0.01 versus PBS treated mice. (D) Proliferation of progenitor cells in WT and CGD mice during acid-elicited acute lung injury was determined by EdU incorporation. Shown are the numbers of indicated progenitor cells in each femur. Data are means ± SD of n=5 mice. * p<0.01 versus PBS treated mice. (E) The number of myeloid progenitors analyzed using an in vitro CFU-GM colony-forming assay. Data shown are mean ± SD of n=5 mice. * p<0.01 versus PBS treated WT mice.