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. Author manuscript; available in PMC: 2017 Mar 22.
Published in final edited form as: Immunol Cell Biol. 2016 Sep 21;95(3):261–271. doi: 10.1038/icb.2016.95

Figure 4. Combined c-REL and RELA deficiency leads to a developmental block at the T1 stage.

Figure 4

(A) IgM and CD23 expression of CD93+ (AA4.1+) splenic B-cells from mice of the indicated genotypes were analyzed by flow cytometry. Numbers beside gates indicate the percentage of T1 (CD93+IgMhiCD23−), T2 (CD93+IgMhiCD23+), and T3 (CD93+IgMloCD23+) B-cells (left). Summary of the frequencies of T1-T3 B-cells (right). Data are cumulative from independent experiments (n=4-9 per group), with each symbol representing a mouse. Data are shown as mean ± standard deviation. Statistical significance was determined by Student's t test (*, P<0.05; **, P<0.01; ***, P<0.001). (B) The fractions of eGFP+ cells among splenic T1 B-cells in relfl/flrelafl/flCD19-Cre and relfl/+relafl/+CD19-Cre mice were determined by flow cytometry. Numbers below gates indicate the percentage of eGFP+ B-cells among the indicated B-cell subsets (left). Data are cumulative from independent experiments (n=4-9 per group), with each symbol representing a mouse, showing the frequency of eGFP+ cells among the corresponding B-cell subsets (right).