Fig. 2.
Crystal structure of human A132L mutant PD-1 shows similar overall architecture to mouse PD-1. (A) Human PD-1 IgV structure. (B) Superposition of human and mouse (RMSD 1.3 for 100 Cα). (C) Mouse PD-1 IgV structure. (D) Superposition of human PD-1 structure with the mPD-1/huPD-L1 complex (pdb ID 3BIK), RMSD 1.1 Å for 100 Cα. (E) Superposition of the human PD-1 structure with mouse PD-1/PD-L2 complex (Pdb ID 3BP5), RMSD 1.1 for 98 Cα. (F) Model of the interface between human (A132L) and mouse PD-1 interacting with human PD-L1. (G) Details of the interactions of human (A132L) and mouse PD-1 with PD-L2. Red: human PD-1 A132L, green: mouse PD-1, cyan: human PD-L1, blue: mouse PD-L2. Dotted lines indicate hydrogen bonds.