Table 2.
Equilibrium dissociation constants (Kds) for the interactions between PD-Ligands, and human and mouse PD-1 and their high-affinity A132L mutants. Kds were determined by non-linear fitting of SPR data, using immobilized PD-Ligands and soluble monomeric wild type or mutant PD-1. Kd values are in μM concentration, followed by the standard errors of the fittings.
| huPD-1 | huA132L | mPD-1 | mA132L | |
|---|---|---|---|---|
| huPD-L1 | 6.36 ± 0.57 | 0.14 ± 0.01 | 4.02 ± 0.06 | 0.48 ± 0.02 |
| huPD-L2 | 0.19 ± 0.019 | 0.0065 ± 0.0014 | 10.69 ± 0.83 | 1.18 ± 0.08 |
| mPD-L1 | 3.81 ± 0.08 | 0.23 ± 0.01 | 5.43 ± 0.08 | 2.78 ± 0.03 |
| mPD-L2 | 0.27 ± 0.01 | 0.029 ± 0.004 | 2.80 ± 0.15 | 0.47 ± 0.02 |