Fig. 5.

Decrease in GAPDH expression induced by PIO in the Hq mouse muscle and cerebellum can be inversely ranked according to their glycaemia status.

(A) Western blot analysis of GAPDH in skeletal muscle or in (B) cerebellum homogenates from 6 month-old untreated WT, Hq mice and 5 months PIO-treated Hq mice. Protein extracts from PIO-treated mice were loaded according to their glycaemia status (ascending from the left). VDAC was indicated as a loading control. Numbers below the right panels refer to mouse numbering as in Fig. 3A. (C) GAPDH, ratioed to VDAC, in the skeletal muscle or in (D) the cerebellum of untreated 6 month-old WT (black open squares) and Hq (black circles), and in 5 months PIO-treated (green circles) 6 month-old Hq mice. PIO-treated Hq animals were also pooled according to their glycaemia, low (green filled crosses) or similar to PIO-treated WT (green open crosses). Biochemical analyses and statistical tests as described under Materials and Methods.