Figure 2.

Representative western blot analysis of multiple NHEJ proteins in cells from control and radiosensitive cancer patients. Each vertical lane represents multiple Western blot analyses from an individual cancer patient. In the demonstrated experiment, cells from 6 control, and 5 radiosensitive cases, are shown (horizontal bars). The analysed NHEJ proteins are shown on the left of the figure (XRCC4, DNA Ligase IV, Ku70 and Ku80), whilst the actin controls for each protein blot are shown on the right. Asterisks show low levels of XRCC4 and DNA Ligase IV in cells from the same patient (RS33). The experiment was performed with over five replicates, and low XRCC4/DNA Ligase IV levels were invariantly seen for RS33 but no other cases. NHEJ, non-homologous end-joining.