Fig. 4.

Effect of GSK461364 on cell cycle progression during the first mitosis in porcine embryos. a Immunofluorescent images were used in porcine embryo cultured with or without GSK461364. After in vitro culture for 48 h, most of the control embryos reached the cytokinesis stage, while the majority of Plk1-inhibited embryos were arrested at the prometaphase stage. Blue, chromosome. Scale bar, 25 μm. b The proportion of embryos at different stages. Most embryos were arrested at the prometaphase stage after Plk1 inhibition. Compared to the control group, the percentage of Plk1-inhibited embryos that finished the first cleavage was sharply decreased, whereas the proportion of embryos which were arrested at the prometaphase stage was significantly increased. *P < 0.05, **P < 0.01, ***P < 0.001